Single-Base Changes: Base Editing
Efficient A-to-G conversions with six stocked ABE8e mRNAs.
- Wild type and high-specificity V106W variants
- NGG, NGN, and NRN PAM options
- Editing shown in HEK293, iPSC, K562, and primary T cells
CRISPR Base & Prime Editing mRNA
Precise genome edits without double-strand DNA breaks
Aldevron, in partnership with IDT
Choose your editing approach
Substitutions, Insertions, Deletions: Prime Editing
PE Ultra mRNA was engineered for improved editing activity compared to PEmax.
- All base substitutions, small insertions, and deletions
- Pairs with IDT Alt-R™ pegRNAs
- Compatible with electroporation and LNP delivery
See the Data
Base Editing
On target vs bystander conversion of various ABE editors in HEK293 cells
Figure 1. Target adenine editing efficiency across cell types for ABE8e-Cas variant combinations. Target adenine conversion at the indicated genomic loci was measured in HEK293, iPSC, K562, and primary T cells using ABE8e editors paired with nCas9, nSpG, or nSPRY. Data from ABE8e-WT and ABE8e-V106W were combined due to comparable on-target editing efficiencies and are presented as mean ± SEM (N = 6 biological replicates; 3 WT and 3 V106W). Cells were nucleofected using the Lonza nucleofection system with 1,000ng of base editor mRNA in HEK293, K562, and primary T cells or 500ng in iPSCs. gRNA was delivered at 2 µM in HEK293, K562, and iPSCs and 4 µM in primary T cells. High levels of target base conversion were observed across diverse cell types and Cas variants.
Prime Editing
Comparison of editing efficiency of PE Ultra mRNA to commercially available PEmax
Figure 1. Editing efficiency of PE Ultra and PEmax in HEK293 cells. PE Ultra and PEmax mRNA and Alt-R pegRNA (6 µM) targeting a broad panel of sites (all 6 bp insertions) were delivered by nucleofection into HEK293 cells using the Lonza Nucleofection® System. Genomic DNA (gDNA) was extracted 72 hours post-nucleofection and editing was assessed by NGS.